FM1-43 DYE
GENERAL CELL SIGNALING
ENDO VS. EXOCYTOSIS
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FM1-43 dye:

FM1-43 Is a styrylpyridinium molecule, more concisely known as a styryl molecule or styryl dye. It is a part of a large family of compounds that were developed as voltage sensitive dyes.

FM1-43 is an ampiphillic molecule, which we know from Bio 181 to be a molecule that has both a hydrophilic and a hydrophobic region. This is what we see in lipid bilayers, and the concept for this molecule is much the same. FM1-43 has a lipophilic tail made up of 2 hydrocarbon chains (something like CH3CH2CH2CH2 etc. etc.), and a positively charged head. The head is a pyridinium group, and it is made up of two aromatic rings with a double bond bridge in between them; this is what is known as the fluorophore part of the dye molecule. This fluorophore group has excitation at 500 nanometers and emission of light at 625 nanometers.

The tail of the FM1-43 molecule is what is really important because this is what allows the dye to get into the plasma membrane of a cell (or in the case of our paper, a synaptic vesicle) because the positively charged head canít get into the lipid bilayer. The interaction of the hydrocarbon tail is what causes the change in brightness.

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