
| Term | Definition |
|---|---|
| atomic-level forcefield | describes the potential energy of a system of particles (atoms). Used to determine efficacy of protein structure when using Monte Carlo Sampling. |
| canonical | repetitive; repeating in different forms |
| chimaera | substance created from genes of two different species |
| cleavage | cutting; splitting |
| cognate | of similar nature |
| computational | designed using a computer |
| dihidrel | formed by two intersecting planes |
| endonuclease | cleaves nucleic acid at certain points to form fragments |
| electron density map | a product of x-ray crystallography. Gives “clouds” of electron energy. From this, atoms and molecules can be placed in the correct orientation because of where their electrons would be positioned. |
| homodimeric | two of the same molecules making one larger molecule |
| In silico screen | using computer programs to alter protein residues and structures. This eliminates the direct need for experimentally modifying protein sequences. |
| intron-encoded | the information can be found in the “useful” DNA |
| In Vitro cleavage assays | this protein is an enzyme that cleaves DNA at specific sites. To test its efficacy, it was placed in a test tube with cells containing DNA on which it could work. |
| Monte Carlo sampling | examining many different residues and orientations of amino acids in a given protein. This allows for determining the best possible structure of the protein. |
| murine hepatocytes | liver tissue cells in mice |
| specificity | at many levels, certain structures determine very specific functions |
| wild-type | reference type; non-mutant form of a gene |
| X-ray crystallography | when an x-ray is passed through a crystal, the beams are scattered because of the positions of the electrons in the crystal. This helps determine the position of the atoms in the crystal along with the bonds that are present. |
Lauren Wiegand
definitions of terms provided by Dr. Florence Tama
MCB 181H- Group 8
The University of Arizona
November 15, 2007
http://student.biology.arizona.edu/honors2007/group08/home08.html
All contents copyright © 2007. All rights reserved.
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