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DNA

Term
Definition
atomic-level forcefield

describes the potential energy of a system of particles (atoms).  Used to determine efficacy of protein structure when using Monte Carlo Sampling.

canonical
repetitive; repeating in different forms
chimaera
substance created from genes of two different species
cleavage
cutting; splitting
cognate
of similar nature
computational
designed using a computer
dihidrel
formed by two intersecting planes
endonuclease
cleaves nucleic acid at certain points to form fragments
electron density map

a product of x-ray crystallography. Gives “clouds” of electron energy. From this, atoms and molecules can be placed in the correct orientation because of where their electrons would be positioned.

homodimeric
two of the same molecules making one larger molecule
In silico screen

using computer programs to alter protein residues and structures. This eliminates the direct need for experimentally modifying protein sequences.

intron-encoded
the information can be found in the “useful” DNA
In Vitro cleavage assays
this protein is an enzyme that cleaves DNA at specific sites. To test its efficacy, it was placed in a test tube with cells containing DNA on which it could work.
Monte Carlo sampling

examining many different residues and orientations of amino acids in a given protein. This allows for determining the best possible structure of the protein.

murine  hepatocytes
liver tissue cells in mice
specificity
at many levels, certain structures determine very specific functions
wild-type
reference type; non-mutant form of a gene
X-ray crystallography
when an x-ray is passed through a crystal, the beams are scattered because of the positions of the electrons in the crystal. This helps determine the position of the atoms in the crystal along with the bonds that are present.

Lauren Wiegand

definitions of terms provided by Dr. Florence Tama

 

MCB 181H- Group 8
The University of Arizona
November 15, 2007

http://student.biology.arizona.edu/honors2007/group08/home08.html
All contents copyright © 2007. All rights reserved.

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