(co2 charts)
These charts show the concentration (percent)of
injected radioactive label in the CO2 collected from middle (left) and
late (right) stage pharate adults injected with labeled arylphorin (Ap) or
labeled phenylalanine (Phe). Note the different percent scales for the
two charts (Late Development has a higher percent scale than does Middle
Development). Error bars were too small to show on the graphs. The given
numerical percent values for Ap and Phe in the center of each chart are
cumulative (start to finish) and represent the recovered radioactively
labeled CO2.
From the chart, overall CO2 production is much higher in late
pharate adult stage than middle pharate adult stage for both arylphorin
and phenylalanine.
(body distribution charts)-- These charts show the distribution (in percent) of the radiolabel from both labeled arylphorin and labeled phenylalaline for both middle and late stages into CO2 and various other M.sexta body structures. Graph value bars exist for Ap-middle stage, Phe-middle stage, Ap-late stage, and Phe-late stage--arylphorin-middle stage pharate adult, phenylalanine-middle stage pharate adult, arylphorin-late stage pharate adult, and phenylalanine-late stage pharate adult, respectively. The four charted values are present in each of the following: CO2, CUT, HEAD, ABD, L&W, THOR, and FHO--(percent in...) CO2, cuticle, head, abdomen, legs & wings, thoracic muscle, and fat body/hemolymph/other gut tissues, respectively. Note the difference in percent scale for the CO2, CUT, and HEAD chart in comparison to the ABD, L&W, THOR, and FHO chart. Also of interest is the very high presence of label in the FHO of the middle stage pharate adult injected with labeled arylphorin. This is consistent with less amino acid "turnover" in earlier pharate adult development and larger arylphorin degradation only in the later pharate stage (present in hemolymph until that point). Aside from this value all middle stage arylphorin and middle stage phenylalanine values are comparable, as are the later stage arylphorin and phenylalanine values. The similarity supports the arylphorin as a storage/reserve protein hypothesis.