RESULTS

Distribution of 14C from 14C-Phe or 14C-Arylphorin Arylphorin plays a role in the forming of the adult tissue which develops as the insect develops and continues to mature. To determine what sort of role the arylphorin has on the adult tissue a comparison of the distribution of 14C from labeled phe or labeled arylphorin that was injected into the pharate adult. It was found that the labeled phe differed at the end of adult development in comparison to an earlier time in the middle development stage. To further understand this a comparison of distribution results for middle and late stages of adult development. The amount of 14C in to CO2 was greater in the late stages than the middle stages. In the middle stage of development the expiration of 14CO2 declined by the third day then be the seventh day the amounts increased to the amount measured on the first day, some even exceeded. The labeled arylphorin has a delayed time when compared to the injected phe. This label of CO2 didnít differ in the middle or late stages, the small percentage of radioactivity in CO2 proved that the fate of phe does not have a significant affect in the pharate adult. When a comparison of the distribution of radioactivity in body parts of the adult a difference was noted in the middle and late stages of development. Despite this no differences were shown in the labeled arylphorin and labeled phe. In the middle stage the recovery of 14C from 14C-phe was larger in the legs and wings. There werenít differences noted in late development. The suggestion is that the forming of the cuticle and head were greater in middle than in the late stages. Development of wings and legs was the same for late and middle stages of development. The development of the abdomen and thoracic muscle was greater in the late stage. This was determined by the 1.7 fold-2.6 fold greater accumulation of 14C in these tissues. The thoracic muscle showed 95% radioactivity.

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SCOTT SMORRA, NAT JOHNSON, CRISTINE BERRY, MARINELA RODRIGUEZ, MARY OLIVER, TARA DAVIS, ELYSE EARNEST
GROUP 12 DECEMBER 1, 1996