RESULTS

VACCINES INJECTED Into MICE	Approximate % lysis (90:1 Tcell to Cancer cell ratio)
mut p53 peptide w/ E3 leader sequence mut p53 peptide w/o E3 sequence whole open reading frame of mut p53 vector only (plasmid DNA) E3 leader followed by wildtype gene	60% 38% 28% 17% 15%

This graph represents mice injected with various vectors and "challenged" with tumor cells. Only the mice immunized with the mutant p53 peptide fused to the E3 leader sequence are protected.

Induction of cytotoxic T lymphocytes (CTLs) is most effective in the tumor-bearing mice injected with the mutant p53 fused to the E3 leader sequence. The CD8+ phenotype of T lymphocytes is necessary for the development of CTLs. These results indicate that the mutant p53 peptide combined with the E3 leader sequence is the most effective way of inducing CTL develpoment from CD8+ which specifically lyse the tumor cells in the mice.

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