PCR Experiment Procedure

The group members first collected samples of their own DNA from their cheek cells. Each cell contains the individual's entire genetic make-up or genome. Specific primers were used for the CCR-5 locus. A small portion of the gene which contains the region known to encode the 32bp deletion was amplified and half of the PCR was used to digest it with ECO-R1, a restriction enzyme. Next, the fragments were viewed on a gel. The gel showed that all of the group members are wild-type for the CCR-5. each represents a microliter.

PCRs were performed with

	50 DNAlysac 30 cycles
	10 PCR 10x buffer 93 degrees Celsius 1 minute
	1 Taq polymerase 60 degrees Celsius 1 minute
	2 10mm dNTPs 72 degrees Celsius 1 minute
	1.5 CCR-5 forward primer
	1.5 CCR-5 reverse primer
	34 d H2O

            == 100 all together

ECO-R1 digestion

	50 per were reduced to 17
	2 ECO-R1 10x buffer added along with 1 ECO-R1 enzyme
	Incubated for 1 hour at 37 degrees Celsius